We corroborated a Netrin-1 dependency in the angiogenic response of GDM HUVEC, although notably, the effect was subtle compared to healthy HUVEC, assessing a ~30% and ~40% reduction in angiogenic response when adding the recombinant antibody anti-Netrin-1 2F5 in 5 mM and 25 mM d-glucose culture conditions, respectively. Here, NTN1 is linked to gestational diabetes.