RUNX3 and cancer: While the fluorescence intensity captured by AFI was decreased in the lesions with an aberrant status of the p53 and p16 genes, which are essential for regulating the cell cycle and the proliferation of cancer [24,25,26], the aberrant methylation of Apc, E-cadherin, Runx3, and hMLH1 did not influence the fluorescence intensity of AFI in colon neoplasms.