A series of 47 synthesized chalcone derivatives were in vitro tested for their cytotoxicity to two wild type EGFR (A431 and A549) and two mutant EGFR (H1650 and H1975) cancer cell lines by the MTT assay in comparison with erlotinib and afatinib, as known inhibitors for wild type and mutant EGFR-TK, respectively. This evidence concerns the gene EGFR and cancer.