Among these receptors, Notch1 is dominantly expressed in hepatocytes.44 High Notch1 expression is associated with large tumour size, microvascular invasion and HCC metastasis.45 Notch1 overexpression predicts an unfavourable survival outcome for liver cancer patients.46 Our results showed that manipulation of KK‐LC‐1 was correlated with altered Notch activity. The gene discussed is NOTCH1; the disease is liver cancer.