Although indel and missense mutations were relatively more frequent in tumours with ≤ and > 10% nuclear staining, respectively, the wild-type genotype comprised 50% and 64% of tumours with ≤ and > 10% nuclear staining, respectively.38 In the present study, 53% of CRCs in the p53-no expression group had stop–gain mutations or indels, whereas 80% of CRCs in the p53-strong expression group were characterised by missense mutations, which suggests that the p53-no expression and p53-strong expression statuses are likely to represent loss of function and gain of function, respectively. The gene discussed is TP53; the disease is neoplasm.