CAP1 and breast carcinoma: Using two shRNA constructs S2 and S3 that target independent nucleotide sequences and effectively silenced CAP1 in HeLa and breast cancer cells12,18,24,33, we were able to generate stable clones with efficient CAP1 knockdown in the PANC-1 and AsPC-1 cells, as confirmed in Western blotting (Fig. 2A).