As shown in Fig. 2f, CRC cells cultured in CAFs-CM significantly enhanced the adhesion between CRC cells and monocytes, but this CAFs-mediated adhesion was impaired when anti-IL6 antibody was administered or after transfection with 3# siRNA of VCAM-1, the effect of siRNA was confirmed by western-blot (Fig. 2g). This evidence concerns the gene VCAM1 and colorectal carcinoma.