TIA1 and amyotrophic lateral sclerosis: Furthermore, Opto-G3BP1 localized to spontaneous stress granules induced by expression of ALS mutant proteins (FUS R521C, TDP-43 ΔNLS, TIA1 A381T) even in the absence of blue light activation, demonstrating that the Opto-G3BP1 protein behaves similarly to endogenous G3BP1 (Figure 1—figure supplement 1b).