For example, PAH sequencing for phenylketonuria had a consistently high diagnostic rate of 96% in 52 samples, sequencing of the COL4A3, COL4A4, and COL4A5 genes in Alport syndrome had a diagnostic yield of 48% in 48 samples and the commonly ordered comprehensive hearing loss panel, most commonly ordered as a panel with 2–149 genes had a diagnostic rate of 50% in 173 samples (Table 1). This evidence concerns the gene COL4A4 and phenylketonuria.