We successfully detected them in constructed plasmids, semi-artificial plasmid/human sequences, and real human sequences from PCR-amplified SCA10 locus, PacBio No-Amp sequence of Huntington’s disease locus [23] (Additional file 1: Figure S20), and PacBio whole genome data from a BAFME patient. This evidence concerns the gene ATXN10 and juvenile Huntington disease.