In conclusion, this study presents the first evidence that the combinational use of low-dose 2-DG and MET can markedly inhibit cell proliferation via simultaneously inhibiting glycolysis and oxidative phosphorylation metabolism, reducing intracellular ATP production, activating AMPK activity, and thereby inhibiting the activation of the mTOR proliferation signaling pathway in polycystic kidney epithelial cells. The gene discussed is MTOR; the disease is polycystic kidney disease.