The main source in co-culture for the release of CCL5, and IL-6, CXCL1, CXCL8, CXCL10, and CCL2 was eosinophils and fibroblasts, respectively, and direct contact between eosinophils and fibroblasts was essential for the release of AD-related chemokine CXCL1, CXCL10, CXCL8, and CCL5. This evidence concerns the gene IL6 and Alzheimer disease.