Whether Sirt6 is regulated by NFκB in this system remains to be explored, however, global profiling of p65 binding sites (by ChIP-seq) in TNFα-induced human osteosarcoma U-2 OS cells (Janus et al., 2018) and TNFα-induced or poly(I:C) stimulated Detroit 562 cells (Borghini et al., 2018) did not identify SIRT6 as a NFκB/p65 target gene. This evidence concerns the gene RELA and osteosarcoma.