To verify the findings obtained using the Aa biofilm model, 3S biofilm-Ti—a more clinically relevant model—was prepared using the peri-implantitis-related bacteria P. gingivalis, F. nucleatum, and S. mitis50 in basal mucin medium, which is frequently used as artificial saliva51–53, and subjected to MTT assays. This evidence concerns the gene MUC5AC and Peri-Implantitis.