The three genes with the strongest signal in the prioritisation analyses were TRIM28, FBXW7, and NYNRIN. We did Sanger sequencing to validate protein-truncating variants and rare non-synonymous variants of these genes in probands and any available samples from relatives to further evaluate their status as bona fide Wilms tumour predisposition genes. The gene discussed is NYNRIN; the disease is Wilms tumor.