AMER1 and Wilms tumor: We believe this mutation to be pathogenic because it is absent from public and in-house datasets, it segregates with Wilms tumour in the family, it is predicted to be deleterious by in silico tools, and it is at a crucial residue within the coiled-coil domain of TRIM28 that is reported to interact with AMER1, which is encoded by a gene somatically mutated in Wilms tumour.19