In the present study, we performed a comprehensive comparison between HHLA2 and PD-L1 in terms of the expression pattern, clinical relevance and their associations with tumor infiltrating CD3+, CD8+, CD4 + Foxp3+, CD68+, CD163+ and CD20+ immune cells in a ICC cohort after curative resection. Here, CD4 is linked to intrahepatic cholangiocarcinoma.