Evaluating and comparing the trends between PCOS and control ovaries in PAI-1 expression (Object Area ROI, %) as follicles developed, similar expression/trends were noted in small through to large follicles for the 2 experimental groups however differential expression/trend for the grouped antral and preovulatory size/stage was observed; PCOS mice noted an increased trend in the latter ovarian follicles/stage while a predominantly downward trend was observed for the control mice (Fig. 6). This evidence concerns the gene SERPINE1 and polycystic ovary syndrome.