According to the results of RT-qPCR (Figure 4A) and western blot (Figure 4B), those results confirmed that the LRP1B expression was significantly increased in PC-3 and LnCap cells transfected with miR-500 inhibitor, proving that LRP1B was identified as a target gene of miR-500 in PC cells. The gene discussed is LRP1B; the disease is pachyonychia congenita.