To first evaluate whether the memory CD8+ T cells in the skin were re-circulating or could potentially be tissue-resident, we again infected LCMV-immune mice with VacV-GP33 and on day 40 post-infection, delivered anti-CD8β antibody by intravenous injection for 3 minutes prior to isolating memory P14 CD8+ T cells from the skin and blood (S4A Fig). Here, CD8A is linked to infection.