On day 3 post-infection, memory P14 CD8+ T cells had proliferated in the draining lymph node, but the majority of the cells that trafficked into VacV-infected skin had not proliferated and were CD127+/KLRG1- (Fig 1B–1E), suggesting that memory CD8+ T cells initially trafficked into the skin directly from the circulation and were not “re-primed” in the draining lymph node prior to infiltrating the skin microenvironment. This evidence concerns the gene CD8A and infection.