As expected, the transplanted tumor with the overexpression of SMAD5-AS1 had a smaller volume and lower weight than that in control group (Fig. 4a), while the transplanted tumor with the down-regulation of SMAD5-AS1 had a larger volume and higher weight than that in the control group (Fig. 4b), and the differences were statistically significant (P < 0.05). This evidence concerns the gene SMAD5 and neoplasm.