As shown in Figs. 5a, b, RBCK1 depletion increased the expression of p53 protein level and its target genes, including P21, BTG2 and P53INP1. To confirm the suppressive effect of RBCK1 on p53 signaling, we treated the two RCC cell lines with cisplatin. Here, CDKN1A is linked to renal cell carcinoma.