Although the four KIFs in normal glial cells, the glioblastoma cell line, and glioma tissues showed internal discrepancies to identify the targets as nuclear or cytoplasmic especially between the immunofluorescence and immunohistochemistry, KIF4A, 18A, and 23 were able to be identified in nuclear localization, however, KIF9 was only localized in cytoplasm. Here, KIF4A is linked to central nervous system cancer.