The concentration of soluble PD-L1 eventually increased in mice bearing 1% MC38/PD-L1v242, although the plasma-soluble PD-L1 level was undetectable in mice bearing wild-type PD-L1 overexpressed MC38 (MC38/PD-L1-WT), even when their tumor volume exceeded 1,000 mm3 (Fig. 9 B). The gene discussed is CD274; the disease is neoplasm.