To extend these observations to an in vivo setting, we produced mouse PD-L1 variants (mPD-L1v242 and mPD-L1v178), which have the same human PD-L1 truncation by aberrant splicing (Fig. S4), and overexpressed them in MC38, a cell line from C57BL/6 murine colon adenocarcinoma cells which has been reported to have high PD-L1 expression induced by IFN-γ and a response to the blockade of the PD-1/PD-L1 axis (Juneja et al., 2017). This evidence concerns the gene PDCD1 and colon adenocarcinoma.