To examine the cause of such disruption, we assessed the global activation of caspase-3 and -7 in the spheroids by live-imaging (Fig. 2b and Additional file 2: Movie S1, Additional file 3: Movie S2, Additional file 4: Movie S3) and quantified Annexin V and DAPI staining of tumor cells by flow cytometry (Fig. 2c). This evidence concerns the gene CASP3 and neoplasm.