Figure 1 shows LCN2 gene expression and protein levels in the peri-infarct zone. We found a stepwise increase of LCN2 mRNA (Figure 1A) in brain tissue with a significant induction at 24 and 72 h post stroke. The Western blot (WB) analysis shows a significant increase of LCN2 protein levels only at 72 h after tMCAO (Figure 1B). The gene discussed is LCN2; the disease is Stroke.