To provide the proof of principle that TRIAP1 mediates radiation resistance, the prostate cancer cells PC3 (p53 null), DU145 (p53 mutant) and LNCaP (p53 wild type) were transiently transfected with an expression vector encoding for human GFP-tagged TRIAP1 (Figure 2A). Here, TP53 is linked to Familial prostate cancer.