In this study, we were able to correlate the expression of the most consistently upregulated A-375 and SKMEL-28 melanoma sEV mRNAs (CXCL1, CXCL2, and CXCL8) to CXCL1, CXCL2, and particularly CXCL8 protein production at the sEV source cell level. Here, CXCL1 is linked to melanoma.