These included a high level (log2ratio >3.5) amplicon targeting MDM2 (12q15) and another targeting both Insulin Receptor Substrate 1 (IRS1) and Serine Protease Rhomboid Domain Containing 1 (RHBDD1) (2q36.3) in the 3.0N population present in the seminoma tissue of patient 5 (Fig 3). The gene discussed is IRS1; the disease is seminoma.