The results showed that overexpression of SPINK5 inhibited the protein expression levels of β‐catenin, p‐GSK3β(S9), c‐myc, and cyclin D1 and upregulated p‐β‐catenin (S33/37) in esophageal cancer cells KYSE510 and ECA109 (Figure 4A). The gene discussed is GSK3B; the disease is esophageal cancer.