We found that SPINK5 acted as a tumor suppressor in esophageal cancer to inhibit proliferation, migration, and migration of esophageal cancer cells via inhibiting Wnt/β‐catenin signaling pathway, which provided an inspiration for exploring the mechanism of action of SPINK5 in tumorigenesis and development, and provides a theoretical basis for the search for new therapeutic targets for esophageal cancer. This evidence concerns the gene SPINK5 and neoplasm.