In esophageal cancer cells KYSE510 and ECA109, overexpression of SPINK5 significantly inhibited TOP flash reporter activity (Figure 4C), while knockdown of SPINK5 significantly increased TOP flash reporter activity (Figure 4D), suggesting that overexpression of SPINK5 inhibits Wnt/β‐catenin signaling pathway activity, while SPINK5 knockdown is able to activate Wnt/β‐catenin signaling pathway. The gene discussed is SPINK5; the disease is esophageal cancer.