In the process of tumor development, activation of Wnt/β‐catenin signaling pathway can upregulate cell proliferation and migration‐related target genes, such as c‐myc and cyclin D1, which promote cell proliferation and migration.30 Therefore, we further demonstrated that SPINK5 overexpression could reverse the effect of LiCl treatment, which confirmed that SPINK5 could indeed inhibit the proliferation and migration of esophageal cancer cells by inhibiting the activity of Wnt/β‐catenin signaling pathway. This evidence concerns the gene SPINK5 and neoplasm.