In this study, we found that the protein expression level of SPINK5 was significantly reduced in esophageal cancer tissues relative to normal esophageal tissues, which was consistent with the results of previous mRNA microarray analysis.11 In addition, in vitro studies showed that SPINK5 overexpression significantly inhibited the proliferation, migration, and invasion of esophageal cancer cells. Here, SPINK5 is linked to esophageal cancer.