Transwell migration assay showed that overexpression of SPINK5 significantly inhibited the migration of esophageal cancer cells KYSE510 and ECA109 (Figure 3A), while knockdown of SPINK5 significantly promoted the migration of esophageal cancer cells KYSE510 and ECA109 (Figure 3B). The gene discussed is SPINK5; the disease is esophageal cancer.