TSPY1 and hepatocellular carcinoma: By comparing these DEGs in HCCs to those derived from HuH-7 overexpressing TSPY (Fig. 2a), 49 DEGs were identified to share similar expression patterns, i.e. either up or down, between HuH-7 overexpressing TSPY and TSPY-high group in clinical HCC specimens (Fig. 3d and Additional file 4: Table S3).