The effects of TSPY in HCC were examined in the HCC HuH-7 cells, which were transduced with Tet-On lentiviral vectors overexpressing TSPY-ires-EGFP and EGFP alone under doxycycline-induced conditions, hereby designated as HuH-7-tetON-TSPY and HuH-7-tetON-EGFP respectively (Fig. 1a). This evidence concerns the gene TSPY1 and hepatocellular carcinoma.