In order to determine the effects of TSPY expression on HCC, we overexpressed TSPY in the HCC cell line, HuH-7, and analyzed the differential gene expression patterns between HuH-7 cells expressing and non-expressing the TSPY transgene using transcriptome analysis, and correlated the results with those derived from comparative study between the male HCC cases with high TSPY expression and non-expression (silent) from the Cancer Genome Atlas (TCGA) [27, 41]. Here, TSPY1 is linked to hepatocellular carcinoma.