The results were as follows: EBV-VCA-IgA, EBV-EA-IgA, clinicopathological stages, β-catenin, TCF-4, and survivin protein expression was independent risk factors for OS of NPC; EBV-EA-IgA, clinicopathological stages, β-catenin, TCF-4, and survivin were independent risk factors for DMFS of NPC; Family history, N stage, TNM stage, TCF-4, EBV DNA, and EBV-EA-IgA as independent risk factors for LRFS of NPC; EBV DNA, EBV-VCA-IgA, EBV-EA-IgA, clinicopathological stages, β-catenin, TCF-4, and survivin were independent prognostic risk factors for DFS of NPC (all P < 0.05) (Table 5). The gene discussed is TCF4; the disease is nasopharyngeal carcinoma.