Expression of TCF-4 protein significantly correlated with EBV DNA, EBV-VCA-IgA, EBV-EA-IgA, T stage, N stage, and clinicopathological stages (all P < 0.05), however, there were no significant correlations between NPC patients’ gender, age, smoking history, family history, and histological classification (all P > 0.05). The gene discussed is TCF4; the disease is nasopharyngeal carcinoma.