ANO1 and intrahepatic cholangiocarcinoma: At this concentration, Ani9 had significant effects on the contractions enhanced by TTX (Figs 2B,D and S2B,F), L-NNA (Fig. 3B,D) and ODQ (Fig. 3F,H), making this drug suitable to test the hypothesis that suppression of Ano1 channels in ICC is a viable mechanism of tonic inhibition in the colon.