ANO1 and intrahepatic cholangiocarcinoma: Since Ano1 channels in ICC are activated by Ca2+ release we also reasoned that interfering with Ca2+ release by inhibiting the SERCA pump or SOCE may also indicate an ICC-dependent mechanism, as excitation-contraction coupling in SMCs is primarily dependent upon Ca2+ entry via voltage-dependent Ca2+ channels and not Ca2+ release mechanisms52.