Finally, to investigate RACGAP1-regulated oncogenes and cancer-associated pathways in PDAC, we performed genome-wide expression analyses in PDAC cells after knockdown of RACGAP1. A total of 64 genes were identified as putative targets of RACGAP1. Among these targets, expression of 12 genes (MMP28, CEP55, CDK1, ANLN, S100A14, SLC6A14, TRIM29, TMPRSS4, SERPINB3, CAPN8, MELK and FAR2) was closely associated with a worse prognosis in patients with PDAC. Here, MELK is linked to cancer.