To test whether exogenous TGF-β1 treatment has an effect on survival and growth, five ccRCC cell lines (786-O, Caki-1, Caki-2, MZ1851RC, MZ2733RC) and one pRCC cell line (MZ2858RC) were left untreated or treated with 10 ng/mL TGF-β for 48 to 96 hours, before their cell viability, proliferation and apoptosis was analyzed. The gene discussed is TGFB1; the disease is nonpapillary renal cell carcinoma.