YAP1 and hemoglobin measurement: Five weeks after establishing Yap1-β-catenin driven HB using SB-HTVI, we monitored tumor growth and development using non-invasive 2D and 3D ultrasound (US) imaging to evaluate changes in tumor burden in the same mice over time, producing a more accurate representation of the effects of Rapamycin while reducing the number of animals used for the study.