Comparative study of these two antibodies either alone or in combination with anti-mouse PD-1 antibody in the CT26 mouse colorectal cancer model showed that anti-mouse LAG-3 antibody with minimal Fc effector functions [IgG1 (D265A)] had anti-tumor efficacy, and the one with effector function (IgG2a) had no apparent tumor inhibitory effect (120). This evidence concerns the gene LAG3 and colorectal cancer.