Of the above 35 ‘cancer’ genes up/downregulated after acute RE, we also demonstrated that 12 were downregulated and hypermethylated, including: RUNX1T1, GAB1, ESR1, LAMA3, NANOG, SMO, ANK3, GADD45G, DROSHA, ATM, APAF1 and AGTR1. The gene discussed is APAF1; the disease is cancer.