TBR1 and Alzheimer disease: These findings are consistent with our prior work demonstrating this protocol efficiently generates mixed neural cell cultures with ~50% TBR1-positive glutamate neurons that have the ability to generate trains of action potentials, display spontaneous synaptic activity indicative of synapse formation, and express functional glutamatergic and GABAergic ionotropic receptors, with no difference in the ability of iPSCs from control or AD donors to generate neural cultures24,25.