We further discovered the targeting relationship between XIST and miR-140-5p, and miR-140-5p and ORC1. The cells experiments proved that XIST could affect ORC1 expression by regulating miR-140-5p, and the further experiments determined the role of XIST/miR-140-5p/ORC1 axis in cervical cancer. The gene discussed is XIST; the disease is cervical carcinoma.