Based on mutation analysis using the AmpliSeq HotSpot panel MUG-Mel1 did not harbour any mutation in the most recurrently mutated genes in melanoma such as in a BRAF, NRAS, KIT. However, NF1 mutation (NM 001042492: c.A5233G, p.K1745E) was identified, which could also be detected in the primary tumor, MUG-Mel1 cell line and the two subclones C8 and D5. The gene discussed is BRAF; the disease is neoplasm.