We depleted GLS, GOT2, and ASNS in SOX12-overexpressing SW480 and Caco-2 cells, and upregulated GLS, GOT2, and ASNS in SOX12-knockdown SW620 and LoVo cells to verify the participation of GLS, GOT2, and ASNS in SOX12-mediated CRC cell proliferation and metastasis (Fig. 5a). This evidence concerns the gene GLS and colorectal carcinoma.