However, mutation in H133 or E94 site did not affect ADI1 effects on apoptosis or cell proliferation in LNCaP cells (a prostate cancer cell line), which was quite different from our findings in HCC cell lines, as H133A mutation abolished both functions of ADI1 in cell growth inhibition and apoptosis enhancement (Fig. 3). This evidence concerns the gene ADI1 and hepatocellular carcinoma.