We found that addition of anti-PD-1 to the cocultures did not alter DNT cell cytotoxicity towards lung cancer cell lines H460, XDC137 and A549 natively expressing PD-L1, but significantly increased killing of PD-L1 overexpressing cell line A549-PD-L1 (Additional file 2: Figure S7B). The gene discussed is PDCD1; the disease is lung cancer.