The silver grain distribution in the hippocampal formation/EC, and frontal, parietal, temporal and occipital cortices in AD brains closely matched the laminar distribution of tangles on adjacent slices as revealed by PHF-1 immunostaining rather than the more scattered plaque distribution pattern revealed by Aβ immunostaining (Fig. 6a-b). The gene discussed is PHF1; the disease is Alzheimer disease.