In this study, we found that RFX1 expression was downregulated in CD14+ monocytes of CAD patients, which led to the overexpression of the target gene TLR4 through the recruitment of DNMT1, HDAC1, and SUV39H1 to regulate DNA methylation and histone modifications in the TLR4 promoter. This evidence concerns the gene HDAC1 and coronary artery disorder.