Moreover, we demonstrated that RFX1 deficiency led to the overexpression of TLR4 and the activation of CD14+ monocytes by inducing decreased DNA methylation and histone H3K9 trimethylation and increased H3 and H4 acetylation in the TLR4 promoter region, which were identified in CD14+ monocytes of CAD patients. The gene discussed is TLR4; the disease is coronary artery disorder.