Here, we found the mRNA expression of TLR4 was increased in CD14+ monocytes from the CAD patients (n = 16) compared with those from the non-CAD controls (n = 14) (Fig. 1c), which was negatively correlated with the mRNA expression of RFX1 (Fig. 1d). The sequences of the primers are shown in Additional file 1: Table S1. This evidence concerns the gene RFX1 and coronary artery disorder.