SPTAN1 and neoplasm: In order to gain insights into the role of SPTAN1 in CRC progression, we then used immunohistochemistry to determine the expression of SPTAN1 in tumor tissue (sample images shown in Fig 1C+1F) and (if available) in the corresponding surrounding normal mucosa (sample images shown in Fig 1B+1E), and quantified values using a simple computer-based algorithm in ImageJ (see Materials and methods).