To investigate whether microglia-derived MV could exert indirect effects on glioma, cells were co-cultured with a mixed neuroglia culture in the presence of LPS/IFNγ-MV, for 18 h: in these conditions a reduction of GL261 viability was observed (Figure 2F), indicating an indirect effect of MV on glioma cell viability. This evidence concerns the gene IFNG and central nervous system cancer.