Until now, some pilot studies have investigated possible correlations of MTHFR rs1801131 (A1298C) and rs1801133 (C677T) polymorphisms with glaucoma, but the results of these studies were conflicting and the sample size of individual studies was relatively small (Al‐Shahrani et al., 2016; Buentello‐Volante et al., 2013; Gupta et al., 2014; Nilforoushan et al., 2012). This evidence concerns the gene MTHFR and glaucoma.