In mouse models for PD-L1 blockade-sensitive colon cancer and PD-L1 blockade-resistant fibrosarcoma32, combined use of ANF and anti-PD-L1 antibody resulted in potentiated T cell infiltration and increased IFNγ and TNFα expression, as well as significant tumor growth inhibition (supplementary Fig. 4, 5). This evidence concerns the gene TNF and colonic neoplasm.