H&E staining showed that B5G1 treatment caused dramatic cell death in the tumor sections, which could be a result of cellular proliferation inhibition and cancer cell apoptosis induction, as the B5G1 group had a lower ki67 index, a cellular marker for proliferation, and higher amount of cleaved caspase-3-positive cells than the vehicle group (Fig. 8d). This evidence concerns the gene CASP3 and cancer.